7KJA

Crystal structure of the EphA2 intracellular KD-SAM domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-11-21
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.5418
Spacegroup name	I 1 2 1
Unit cell lengths	123.060, 55.030, 135.644
Unit cell angles	90.00, 94.86, 90.00

Refinement procedure

Resolution	20.490 - 1.750
R-factor	0.1898
Rwork	0.188
R-free	0.21750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pdo 3kka
RMSD bond length	0.004
RMSD bond angle	0.715
Data reduction software	MOSFLM (version 7.1.0)
Data scaling software	Aimless (version 0.3.11)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.490	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.059	0.690
Rmeas	0.081	0.953
Rpim	0.056	0.654
Number of reflections	91439	4522
<I/σ(I)>	13.4	1.4
Completeness [%]	99.9	100
Redundancy	3.6	3.5
CC(1/2)	0.997	0.599

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	287	0.2 M MgCl2, 0.1 M Bis-Tris pH5.5, 25% PEG 3350