7KIK
Wheat dwarf virus Rep domain circular permutation complexed with a single-stranded DNA 10-mer comprising the cleavage site and Mn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-27 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97910 |
| Spacegroup name | I 4 |
| Unit cell lengths | 94.252, 94.252, 50.107 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.650 - 1.730 |
| R-factor | 0.1574 |
| Rwork | 0.156 |
| R-free | 0.18740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6we0 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.650 | 1.792 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.074 | 0.756 |
| Rmeas | 0.089 | 0.922 |
| Rpim | 0.050 | 0.521 |
| Number of reflections | 22917 | 2283 |
| <I/σ(I)> | 14.14 | 1.8 |
| Completeness [%] | 99.1 | 99.04 |
| Redundancy | 3 | 3.1 |
| CC(1/2) | 0.994 | 0.994 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | 0.1 M Bis-tris, pH 6.2, 10% polyethylene glycol, 20% glycerol added to drop |
