7KIJ
Muscovy duck circovirus Rep domain complexed with a single-stranded DNA 10-mer comprising the cleavage site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-27 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97910 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 102.986, 102.986, 138.294 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 54.650 - 1.690 |
R-factor | 0.1718 |
Rwork | 0.171 |
R-free | 0.19460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6wdz |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.650 | 1.751 |
High resolution limit [Å] | 1.690 | 1.690 |
Rmerge | 0.036 | 0.825 |
Rmeas | 0.039 | 0.878 |
Rpim | 0.013 | 0.292 |
Number of reflections | 48935 | 4791 |
<I/σ(I)> | 32.89 | 2.28 |
Completeness [%] | 99.8 | 99.69 |
Redundancy | 8.7 | 8.5 |
CC(1/2) | 1.000 | 0.784 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 0.1 M sodium acetate, pH 4.8, 2.2M ammonium sulfate, 20% glycerol added to drop |