7KII

Muscovy duck circovirus Rep domain complexed with a single-stranded DNA 10-mer comprising the cleavage site and Mn2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-27
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97910
Spacegroup name	I 2 2 2
Unit cell lengths	55.417, 66.343, 71.259
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.560 - 1.300
R-factor	0.151
Rwork	0.149
R-free	0.18430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6wdz
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.560	1.346
High resolution limit [Å]	1.300	1.300
Rmerge	0.053	0.368
Rmeas	0.059	0.419
Rpim	0.025	0.196
Number of reflections	32195	3197
<I/σ(I)>	12.24	2.18
Completeness [%]	98.4	99.35
Redundancy	4.6	4.3
CC(1/2)	0.998	0.943

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	298	0.1 M sodium acetate, pH 4.8, 20 mM Calcium chloride, 26% 2-methyl-2,4-pentanediol, 30% polyethylene glycol 200 added to drop