7KGV

Crystal structure of sodium-coupled neutral amino acid transporter SLC38A9 in the N-terminal plugged form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	93
Detector technology	PIXEL
Collection date	2017-12-06
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	157.798, 82.514, 158.590
Unit cell angles	90.00, 106.02, 90.00

Refinement procedure

Resolution	49.370 - 3.400
R-factor	0.2519
Rwork	0.251
R-free	0.28430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1f8t
RMSD bond length	0.011
RMSD bond angle	1.325
Data reduction software	XDS (20170601)
Data scaling software	Aimless (0.5.23)
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.370	3.520
High resolution limit [Å]	3.400	3.400
Rpim	0.084	1.071
Number of reflections	54452	5375
<I/σ(I)>	6.5	1
Completeness [%]	99.9	99.8
Redundancy	11.8	6.1
CC(1/2)	0.989	0.350

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	26-30% (w/v) PEG400, 100 mM ADA pH 6.0, 350 mM Li2SO4.