7KFV

Structural basis for a germline-biased antibody response to SARS-CoV-2 (RBD:C1A-B12 Fab)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-07-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	84.834, 113.250, 268.878
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	134.440 - 2.102
R-factor	0.1846
Rwork	0.183
R-free	0.21550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7bz5
RMSD bond length	0.008
RMSD bond angle	0.970
Data reduction software	XDS (20200131)
Data scaling software	XDS (20200131)
Phasing software	PHASER (2.8.3)
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	200.000	2.230
High resolution limit [Å]	2.100	2.100
Rmeas	0.257
Number of reflections	149225	44734
<I/σ(I)>	8.98	1.35
Completeness [%]	98.9	94.7
Redundancy	7.01	6.4
CC(1/2)	0.995	0.397

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293.15	protein sample: 13 mg/mL in 150mM NaCl, 25 mM Tris-HCl, pH 7.5 mother liquor composition (equal volume): 0.1 M BICINE pH 8.5, 20% (w/v ) PEG 10000