7KFP
Structure of human PARG complexed with PARG-119
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-26 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9201 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.532, 88.660, 94.426 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.580 - 1.900 |
R-factor | 0.1757 |
Rwork | 0.175 |
R-free | 0.19530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4b1g |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.580 | 1.968 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.087 | 0.669 |
Rmeas | 0.095 | 0.734 |
Rpim | 0.039 | 0.298 |
Number of reflections | 44680 | 4419 |
<I/σ(I)> | 10.52 | 2.25 |
Completeness [%] | 99.9 | 99.98 |
Redundancy | 5.9 | 6 |
CC(1/2) | 0.997 | 0.760 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.1 M PCTP, pH 7.5, 0.2 M AmSO4, 18-23% PEG3350 |