7KFP

Structure of human PARG complexed with PARG-119

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-26
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9201
Spacegroup name	P 21 21 21
Unit cell lengths	66.532, 88.660, 94.426
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.580 - 1.900
R-factor	0.1757
Rwork	0.175
R-free	0.19530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4b1g
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.580	1.968
High resolution limit [Å]	1.900	1.900
Rmerge	0.087	0.669
Rmeas	0.095	0.734
Rpim	0.039	0.298
Number of reflections	44680	4419
<I/σ(I)>	10.52	2.25
Completeness [%]	99.9	99.98
Redundancy	5.9	6
CC(1/2)	0.997	0.760

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	0.1 M PCTP, pH 7.5, 0.2 M AmSO4, 18-23% PEG3350