7KFK
Crystal structure of LILRB1 D3D4 domain in complex with Plasmodium RIFIN (PF3D7_1373400) V2 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-25 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 111.044, 111.044, 152.124 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.440 - 2.630 |
R-factor | 0.2291 |
Rwork | 0.227 |
R-free | 0.27080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lla |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (dev_3965) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.700 |
High resolution limit [Å] | 2.630 | 7.190 | 2.650 |
Rmerge | 0.202 | 0.070 | 1.306 |
Rmeas | 0.207 | 0.073 | 1.346 |
Rpim | 0.045 | 0.019 | 0.322 |
Number of reflections | 28673 | 1551 | 1410 |
<I/σ(I)> | 5.2 | ||
Completeness [%] | 99.8 | 96.2 | 99.9 |
Redundancy | 21.2 | 14.5 | 16.9 |
CC(1/2) | 0.998 | 0.896 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Tris 8.3, 11% PEG3350, 1.1mM AmSO4, 0.2mM Li2SO4 |