7KF4
Crystal structure from SARS-CoV-2 NendoU NSP15
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.976254 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 85.125, 151.163, 199.242 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.160 - 2.610 |
| R-factor | 0.22 |
| Rwork | 0.219 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6x1b |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.160 | 2.703 |
| High resolution limit [Å] | 2.610 | 2.610 |
| Number of reflections | 78886 | 7756 |
| <I/σ(I)> | 8.24 | |
| Completeness [%] | 99.7 | |
| Redundancy | 13.8 | |
| CC(1/2) | 0.998 | 0.527 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 293 | 0.1 M trisodium citrate pH 5, 14 % w/v PEG6000 |
