7KEE
RNA polymerase II elongation complex with unnatural base dTPT3, rNaMTP bound to E-site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-03-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 169.544, 222.739, 194.661 |
Unit cell angles | 90.00, 101.90, 90.00 |
Refinement procedure
Resolution | 49.150 - 3.450 |
R-factor | 0.2595 |
Rwork | 0.259 |
R-free | 0.30190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2e2j |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.3) |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.155 | 49.150 | 3.470 |
High resolution limit [Å] | 3.410 | 18.680 | 3.410 |
Rmerge | 0.657 | 0.047 | 3.320 |
Rmeas | 0.710 | 0.051 | 3.596 |
Rpim | 0.267 | 0.019 | 1.369 |
Total number of observations | 4217 | 32004 | |
Number of reflections | 95996 | 584 | 4693 |
<I/σ(I)> | 4.1 | 34.4 | 0.7 |
Completeness [%] | 99.9 | 94.7 | 100 |
Redundancy | 7 | 7.2 | 6.8 |
CC(1/2) | 0.948 | 0.998 | 0.241 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 5mM dioxane, 5mM DTT, 10-12% PEG6000 |