7K6R

Crystal structure of the bromodomain (BD) of human Bromodomain and PHD finger-containing Transcription Factor (BPTF) bound to GSK4027

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 1
Unit cell lengths	27.060, 35.520, 41.150
Unit cell angles	114.12, 95.93, 91.06

Refinement procedure

Resolution	37.280 - 1.600
R-factor	0.1636
Rwork	0.161
R-free	0.19720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3uv2
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.280	37.280	1.640
High resolution limit [Å]	1.600	7.160	1.600
Rmerge	0.035	0.021	0.218
Rmeas	0.050	0.030	0.308
Total number of observations	31539
Number of reflections	17236	187	1262
<I/σ(I)>	12.84	30.97	3.37
Completeness [%]	93.8	92.1	93.5
Redundancy	1.83	1.898	1.898
CC(1/2)	0.998	0.998	0.866

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 M Lithium sulfate monohydrate, 0.1M BIS-TRIS, 25 percent Polyethylene glycol 3,350