7K3J

Crystal structure of dLC8 in complex with Panoramix TQT+TQ peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-24
Detector	PSI PILATUS 6M
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	38.100, 175.230, 51.020
Unit cell angles	90.00, 105.48, 90.00

Refinement procedure

Resolution	49.169 - 2.500
R-factor	0.2505
Rwork	0.249
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3zke
RMSD bond length	0.003
RMSD bond angle	0.686
Data reduction software	iMOSFLM
Data scaling software	SCALA (3.3.22)
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	87.615	49.169	2.640
High resolution limit [Å]	2.500	7.910	2.500
Rmerge		0.059	0.443
Rmeas	0.081	0.065	0.484
Rpim	0.032	0.026	0.190
Total number of observations	137634	4373	20005
Number of reflections	21856	692	3184
<I/σ(I)>	12.8	26.9	3.5
Completeness [%]	98.2	95.6	98.4
Redundancy	6.3	6.3	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.2 M Li2SO4, 0.1 M Tris pH 8.5, 40% (v/v) PEG 400