7JYD
Human Liver Receptor Homolog-1 in Complex with 10CA and a Fragment of Tif2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2018-12-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 88.370, 88.370, 105.674 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.770 - 2.300 |
| R-factor | 0.191 |
| Rwork | 0.189 |
| R-free | 0.22730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5l11 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.518 |
| Data reduction software | PHENIX |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.12-2829-000) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
| Rmerge | 0.171 | 0.151 | 1.164 |
| Rmeas | 0.177 | 0.157 | 1.204 |
| Rpim | 0.045 | 0.041 | 0.305 |
| Total number of observations | 343073 | ||
| Number of reflections | 21672 | 2315 | 2133 |
| <I/σ(I)> | 5.9 | ||
| Completeness [%] | 100.0 | 99.8 | 100 |
| Redundancy | 15.8 | 14.3 | 15.3 |
| CC(1/2) | 0.991 | 0.776 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.4 M sodium/potassium tartrate |
