7JXU
Structure of monobody 32 human MLKL pseudokinase domain complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-05 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | P 43 |
Unit cell lengths | 94.619, 94.619, 115.197 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.200 | 49.200 | 2.540 |
High resolution limit [Å] | 2.440 | 8.800 | 2.440 |
Rmerge | 0.102 | 0.036 | 1.457 |
Rmeas | 0.110 | 0.039 | 1.578 |
Rpim | 0.041 | 0.015 | 0.604 |
Total number of observations | 266899 | 5792 | 27806 |
Number of reflections | 37642 | 832 | 4134 |
<I/σ(I)> | 9.5 | 34.4 | 1 |
Completeness [%] | 99.7 | 99.2 | 97.6 |
Redundancy | 7.1 | 7 | 6.7 |
CC(1/2) | 0.995 | 0.992 | 0.597 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 281.15 | 0.1 M Bis-Tris chloride, pH 6.5, 20 % w/v PEG monomethyl ether 5000 |