7JXS

Crystal Structure Human Immunodeficiency Virus-1 Matrix protein Mutant Q63R Crystal Form 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	64.548, 90.724, 73.169
Unit cell angles	90.00, 102.39, 90.00

Refinement procedure

Resolution	45.360 - 2.350
R-factor	0.2184
Rwork	0.216
R-free	0.25430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hiw
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.360	2.434
High resolution limit [Å]	2.350	2.350
Rmerge	0.072	0.431
Rmeas	0.088	0.515
Rpim	0.050	0.277
Number of reflections	32585	3286
<I/σ(I)>	25.95
Completeness [%]	94.5	95.36
Redundancy	3.1
CC(1/2)	0.987	0.807

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4	293	40 % PEG 400, 0.1 M NaAcetate (pH 4.0), 0.05 M Li2SO4