7JWP
Fab CJ11 in complex IL-18 peptide liberated by Caspase cleavage
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2017-08-31 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 81.348, 83.524, 145.794 |
| Unit cell angles | 107.24, 106.53, 89.98 |
Refinement procedure
| Resolution | 23.972 - 3.000 |
| R-factor | 0.2507 |
| Rwork | 0.235 |
| R-free | 0.26040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5i8o |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.998 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (dev_2747) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.060 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.090 | 0.100 |
| Number of reflections | 60390 | 500 |
| <I/σ(I)> | 6.5 | |
| Completeness [%] | 86.4 | |
| Redundancy | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 293 | 0.1 Na citrate, pH 6, 20% PEG4000, 20% isopropanol; Fab was reductively methylated |
