7JW7
Structure of monobody 27 human MLKL pseudokinase domain complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-05 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 64.805, 64.805, 226.763 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.350 | 45.350 | 2.740 |
High resolution limit [Å] | 2.630 | 9.100 | 2.630 |
Rmerge | 0.114 | 0.030 | 1.714 |
Rmeas | 0.120 | 0.032 | 1.803 |
Rpim | 0.038 | 0.011 | 0.554 |
Total number of observations | 171651 | 4108 | 20976 |
Number of reflections | 17332 | 498 | 2048 |
<I/σ(I)> | 11.1 | 38.7 | 1.3 |
Completeness [%] | 99.8 | 99.1 | 99 |
Redundancy | 9.9 | 8.2 | 10.2 |
CC(1/2) | 0.999 | 0.999 | 0.916 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 281.15 | 0.2 M magnesium chloride, 20% w/v PEG 3350 |