7JV7
Crystal Structure of the yeast RNA Pol II CTD kinase CTDK-1 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-10-19 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.004 |
Spacegroup name | P 1 |
Unit cell lengths | 55.170, 71.091, 84.797 |
Unit cell angles | 79.08, 86.22, 72.69 |
Refinement procedure
Resolution | 29.242 - 1.851 |
R-factor | 0.184364153002 |
Rwork | 0.184 |
R-free | 0.22173 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.865 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.880 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.068 | 0.530 |
Number of reflections | 91889 | 4321 |
<I/σ(I)> | 15.6 | |
Completeness [%] | 89.4 | |
Redundancy | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 293 | 0.1 M Sodium Citrate pH 5.3, 0.3 M Lithium Chloride, and 17.5% PEG3350 |