7JQM

Crystal structure of the Thermus thermophilus 70S ribosome in complex with Bac7-002, mRNA, and deacylated P-site tRNA at 3.05A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-29
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97910
Spacegroup name	P 21 21 21
Unit cell lengths	208.640, 447.570, 619.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	151.790 - 3.050
R-factor	0.2295
Rwork	0.227
R-free	0.28340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4p
RMSD bond length	0.004
RMSD bond angle	0.809
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	151.790	151.790	3.130
High resolution limit [Å]	3.050	13.640	3.050
Rmerge	0.281	0.051	1.448
Rmeas	0.334	0.060	1.726
Total number of observations	3726689
Number of reflections	1075967	12145	78327
<I/σ(I)>	4.21	15.98	0.91
Completeness [%]	98.8	93.6	97.7
Redundancy	3.464	3.31	3.348
CC(1/2)	0.982	0.997	0.178

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME