7JPH
Crystal structure of EBOV glycoprotein with modified HR1c and HR2 stalk at 3.2 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 114.061, 114.061, 136.216 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.439 - 3.195 |
| Rwork | 0.277 |
| R-free | 0.32160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jq3 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.799 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.439 | 3.310 |
| High resolution limit [Å] | 3.195 | 3.200 |
| Number of reflections | 17343 | 864 |
| <I/σ(I)> | 8.4 | 0.45 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 8.9 | |
| CC(1/2) | 0.776 | 0.313 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 0.1 M Sodium citrate, 10% PEG w/v 6000 |
