7JPD

Crystal structure of the trimeric full length mature hemagglutinin from influenza A virus A/Fort Monmouth/1/1947

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-02
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	131.790, 142.360, 234.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.850 - 2.950
R-factor	0.2554
Rwork	0.254
R-free	0.28640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vli
RMSD bond length	0.004
RMSD bond angle	0.671
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (dev-4274)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.850	48.850	3.030
High resolution limit [Å]	2.950	13.190	2.950
Rmerge	0.145	0.038	0.828
Rmeas	0.159	0.041	0.894
Total number of observations	573436
Number of reflections	91660	1142	6805
<I/σ(I)>	9.41	23.46	2
Completeness [%]	98.2	97.4	100
Redundancy	6.256	5.982	6.967
CC(1/2)	0.996	0.999	0.715

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	287	InvbN.18715.a.KN11.PD38312 at 9.84 mg/mL against MCSG1 screen condition D11: 0.2 M sodium iodide, 20% PEG 3350 supplemented with 20% ethylene glycol as cryo-protectant; crystal tracking ID 301393d11, unique puck ID umg7-8