Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-14 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 48.967, 48.967, 80.278 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.410 - 1.880 |
| R-factor | 0.1911 |
| Rwork | 0.187 |
| R-free | 0.22440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ZAI |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_3946) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.910 |
| High resolution limit [Å] | 1.880 | 5.100 | 1.880 |
| Rpim | 0.027 | ||
| Number of reflections | 9412 | 546 | 411 |
| <I/σ(I)> | 20.12 | ||
| Completeness [%] | 99.4 | ||
| Redundancy | 4.8 | ||
| CC(1/2) | 1.000 | 0.999 | 0.754 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 295 | 0.1 M Sodium chloride 0.1 M BIS-TRIS propane pH 9.0, 25% w/v Polyethylene glycol 1,500. Crystals formed within a couple days |
