7JMK

GTP-specific succinyl-CoA synthetase complexed with Mg-GDP in space group P32

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 32
Unit cell lengths	99.762, 99.762, 134.206
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	53.000 - 2.500
R-factor	0.2415
Rwork	0.240
R-free	0.26940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fp4
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (dev_3885)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.650	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.088	0.528
Rpim	0.045	0.259
Number of reflections	51719	2593
<I/σ(I)>	8.7	0.4
Completeness [%]	100.0
Redundancy	4.8
CC(1/2)	0.997	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	1.0 M sodium citrate, Tris-HCl pH 7.4