7JKP

Human PrimPol misinserting dATP opposite the 8-oxoguanine lesion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-19
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979200
Spacegroup name	P 1
Unit cell lengths	52.225, 65.656, 74.482
Unit cell angles	69.30, 82.87, 88.06

Refinement procedure

Resolution	69.170 - 2.592
R-factor	0.2504
Rwork	0.248
R-free	0.30660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5l2x
RMSD bond length	0.011
RMSD bond angle	0.495
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.170	2.690
High resolution limit [Å]	2.580	2.580
Rmerge	0.106
Number of reflections	27189	2898
<I/σ(I)>	7.6
Completeness [%]	94.0
Redundancy	3.4
CC(1/2)	0.995	0.523

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	225- 250 mM CaCl2 16 -19% PEG 3350