7JK7

Crystal structure of Importin alpha 2 in complex with LSD1 NLS S111E mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9357
Spacegroup name	P 21 21 21
Unit cell lengths	98.320, 78.630, 89.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.684 - 1.960
R-factor	0.1825
Rwork	0.181
R-free	0.20630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bvt
RMSD bond length	0.007
RMSD bond angle	0.825
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.684	1.960
High resolution limit [Å]	1.900	1.960
Rmerge	0.041	1.236
Number of reflections	48384	3645
<I/σ(I)>	13.5
Completeness [%]	96.5
Redundancy	3.1
CC(1/2)	0.999	0.583

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.7 M sodium citrate, 0.01M DTT, 0.1M sodium HEPES pH 7