7JJL
Crystal structure of Importin Alpha 3 in complex with human LSD1 NLS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9357 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.920, 89.120, 143.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.310 - 2.600 |
R-factor | 0.2071 |
Rwork | 0.204 |
R-free | 0.26610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6bvz |
Data reduction software | Aimless |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (v1.12) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.310 | 2.600 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.493 | |
Number of reflections | 19536 | 2323 |
<I/σ(I)> | 2.1 | |
Completeness [%] | 99.5 | |
Redundancy | 3.7 | |
CC(1/2) | 0.997 | 0.778 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.7 M sodium citrate, 0.01M DTT, 0.1M sodium HEPES pH 7 |