7JHS
Self-assembly of a 3D DNA crystal lattice (4x6 junction version) containing the J5 immobile Holliday junction with R3 symmetry
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.92 |
Spacegroup name | H 3 |
Unit cell lengths | 114.779, 114.779, 49.623 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.116 - 3.096 |
R-factor | 0.2602 |
Rwork | 0.259 |
R-free | 0.26870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6xna |
RMSD bond length | 0.005 |
RMSD bond angle | 0.740 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.150 |
High resolution limit [Å] | 3.096 | 8.400 | 3.100 |
Rmerge | 0.119 | 0.132 | 0.594 |
Rmeas | 0.125 | 0.138 | 0.634 |
Rpim | 0.038 | 0.041 | 0.216 |
Number of reflections | 4170 | 221 | 121 |
<I/σ(I)> | 5.4 | ||
Completeness [%] | 94.0 | 100 | 65.8 |
Redundancy | 9.3 | 10.7 | 6 |
CC(1/2) | 0.972 | 0.932 | 0.955 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.5 mL of 0.05 M Cacodylate pH 6.5 with 10 mM MgCl2, 2.5 mM spermine, 1 mM CuSO4, and 10% Isopropanol was added to the reservoir with 2 uL added to the drop containing 4 uL of DNA stock |