7JHO

Structure of human beta 1,3-N-acetylglucosaminyltransferase 2 with UDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	87
Detector technology	PIXEL
Collection date	2017-06-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.00
Spacegroup name	C 1 2 1
Unit cell lengths	137.651, 76.511, 82.704
Unit cell angles	90.00, 109.93, 90.00

Refinement procedure

Resolution	29.220 - 1.850
R-factor	0.1671
Rwork	0.165
R-free	0.21110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7jhi
RMSD bond length	0.006
RMSD bond angle	0.901
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.880
High resolution limit [Å]	1.850	5.010	1.850
Rmerge	0.087	0.041	1.022
Rmeas	0.094	0.045	1.143
Rpim	0.037	0.017	0.499
Number of reflections	68984	3557	3401
<I/σ(I)>	7.5
Completeness [%]	99.8	100	97.7
Redundancy	6.4	6.5	4.9
CC(1/2)		0.998	0.594

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	24% PEG1500, 20% glycerol