7JHN
Structure of human beta 1,3-N-acetylglucosaminyltransferase 2 with UDP and trisaccharide GlcNAc-beta1-3Gal-beta1-4GlcNAc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 87 |
Detector technology | CCD |
Collection date | 2017-11-29 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 82.916, 76.865, 66.786 |
Unit cell angles | 90.00, 105.82, 90.00 |
Refinement procedure
Resolution | 39.894 - 2.200 |
R-factor | 0.1984 |
Rwork | 0.196 |
R-free | 0.25510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7jhi |
RMSD bond length | 0.004 |
RMSD bond angle | 0.687 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.240 |
High resolution limit [Å] | 2.200 | 5.970 | 2.200 |
Rmerge | 0.050 | 0.030 | 0.506 |
Rmeas | 0.058 | 0.036 | 0.611 |
Rpim | 0.030 | 0.019 | 0.337 |
Number of reflections | 17836 | 1062 | 526 |
<I/σ(I)> | 11.2 | ||
Completeness [%] | 86.7 | 99.8 | 51.3 |
Redundancy | 3.5 | 3.7 | 2.7 |
CC(1/2) | 0.998 | 0.749 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 24% PEG1500, 20% glycerol |