7JHL

Structure of human beta 1,3-N-acetylglucosaminyltransferase 2 with UDP-N-acetylglucosamine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	87
Detector technology	PIXEL
Collection date	2017-10-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.00
Spacegroup name	I 1 2 1
Unit cell lengths	82.654, 76.638, 134.206
Unit cell angles	90.00, 105.27, 90.00

Refinement procedure

Resolution	46.420 - 2.260
R-factor	0.1771
Rwork	0.175
R-free	0.22410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7jhi
RMSD bond length	0.004
RMSD bond angle	0.689
Data reduction software	XDS
Data scaling software	Aimless (0.5.29)
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.420	46.420	2.330
High resolution limit [Å]	2.260	9.040	2.260
Rmerge	0.152	0.054	0.978
Rmeas	0.165	0.059	1.057
Rpim	0.063	0.023	0.398
Total number of observations	255698	4175	24126
Number of reflections	37963	633	3470
<I/σ(I)>	9.4	22.2	2.4
Completeness [%]	99.9	99.1	100
Redundancy	6.7	6.6	7
CC(1/2)	0.996	0.997	0.813

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	24% PEG1500, 20% glycerol