7GQS
Crystal Structure of Werner helicase fragment 517-945 in complex with ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-09-15 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.99997 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.071, 90.066, 53.788 |
Unit cell angles | 90.00, 102.46, 90.00 |
Refinement procedure
Resolution | 48.890 - 1.570 |
R-factor | 0.1776 |
Rwork | 0.175 |
R-free | 0.21850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | inhouse model |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (dev_4357) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.890 | 48.890 | 1.610 |
High resolution limit [Å] | 1.570 | 7.030 | 1.570 |
Rmerge | 0.089 | 0.029 | 3.741 |
Rmeas | 0.096 | 0.032 | 4.025 |
Total number of observations | 447065 | ||
Number of reflections | 63541 | 762 | 4628 |
<I/σ(I)> | 10.47 | 46.35 | 0.47 |
Completeness [%] | 98.2 | 99.2 | 96.8 |
Redundancy | 7.036 | 6.757 | 7.275 |
CC(1/2) | 0.999 | 0.999 | 0.216 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 5 mg/mL protein in 20mM TRIS/HCl pH7.5, 350mM NaCl, 0.25mM TCEP, 2.5% glycerol mixed 40nL with 180nL reservoir consisting of 9.091 %w/v Silver Bullets 42, 0.091 M MES monohydrate, 2.727 %v/v Jeffamine T-403, 13.6 %v/v pentaerythritol ethoxylate, 0.182M Potassium acetate pH6 |