7G8V
ARHGEF2 PanDDA analysis group deposition -- ARHGEF2 and RhoA in complex with Z133716556
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92124 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 71.015, 71.015, 196.333 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.780 - 1.451 |
| R-factor | 0.1952 |
| Rwork | 0.194 |
| R-free | 0.21930 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 8bnt |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | REFMAC |
| Refinement software | BUSTER (2.10.4 (17-FEB-2023)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 98.150 | 98.150 | 1.480 |
| High resolution limit [Å] | 1.450 | 7.810 | 1.450 |
| Rmerge | 0.201 | 0.050 | 5.057 |
| Rmeas | 0.205 | 0.052 | 5.218 |
| Rpim | 0.041 | 0.011 | 1.271 |
| Total number of observations | 2169581 | 14705 | 74545 |
| Number of reflections | 89854 | ||
| <I/σ(I)> | 8.7 | 54.4 | 0.3 |
| Completeness [%] | 100.0 | 100 | 99.7 |
| Redundancy | 24.1 | 20.4 | 16.6 |
| CC(1/2) | 0.999 | 0.999 | 0.325 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM Tris, 2.6M sodium formate |
