7FYW

Crystal Structure of apo mouse FABP5, twinned in P21 with beta=90deg

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-04-09
Detector	PSI PILATUS 6M
Wavelength(s)	1.000000
Spacegroup name	P 1 21 1
Unit cell lengths	59.625, 75.128, 61.805
Unit cell angles	90.00, 90.18, 90.00

Refinement procedure

Resolution	42.977 - 1.810
R-factor	0.208
Rwork	0.206
R-free	0.25140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	inhouse model
RMSD bond length	0.013
RMSD bond angle	0.952
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_728)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.980	42.977	1.860
High resolution limit [Å]	1.830	8.090	1.810
Rmerge	0.083	0.040	1.449
Rmeas	0.090	0.045	1.581
Total number of observations	167736
Number of reflections	25292	350	1884
<I/σ(I)>	10.02	24.87	1.29
Completeness [%]	99.2	98.3	99.4
Redundancy	6.36	5.563	6.259
CC(1/2)	0.998	0.998	0.689

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	protein in 25mM Tris/HCl pH 7.5 100mM NaCl, see also PMID 27658368