7FXO
Crystal Structure of apo human FABP1 monoclinic form III
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-04-01 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.999980 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.941, 37.114, 60.491 |
Unit cell angles | 90.00, 113.12, 90.00 |
Refinement procedure
Resolution | 32.320 - 1.300 |
R-factor | 0.1559 |
Rwork | 0.153 |
R-free | 0.20360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | inhouse model |
RMSD bond length | 0.018 |
RMSD bond angle | 1.897 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0018) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 32.320 | 32.320 | 1.330 |
High resolution limit [Å] | 1.320 | 5.810 | 1.300 |
Rmerge | 0.040 | 0.023 | 1.105 |
Rmeas | 0.047 | 0.028 | 1.321 |
Total number of observations | 185836 | ||
Number of reflections | 54768 | 687 | 4216 |
<I/σ(I)> | 11.36 | 39.02 | 1.11 |
Completeness [%] | 99.6 | 96.9 | 98.6 |
Redundancy | 3.28 | 3.371 | 3.231 |
CC(1/2) | 0.999 | 0.999 | 0.384 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | protein in 25mM Tris/HCl pH 7.5 100mM NaCl, see also PMID 27658368 |