7FI3
Archaeal oligopeptide permease A (OppA) from Thermococcus kodakaraensis in complex with an endogenous pentapeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2016-02-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97864 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 122.661, 118.635, 112.718 |
| Unit cell angles | 90.00, 105.32, 90.00 |
Refinement procedure
| Resolution | 19.980 - 2.300 |
| R-factor | 0.162 |
| Rwork | 0.159 |
| R-free | 0.19180 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.728 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.139 | 0.694 |
| Number of reflections | 137907 | 20087 |
| <I/σ(I)> | 12.5 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.9 | |
| CC(1/2) | 0.995 | 0.780 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 1500, MIB (sodium malonate, imidazole, and boric acid) |
