7FH0
Crystallographic structure of two neutralizing nanobodies in complex with SARS-CoV-2 spike receptor-binding Domain (RBD)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-07 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 154.456, 154.456, 257.916 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.500 - 3.200 |
| R-factor | 0.2102 |
| Rwork | 0.207 |
| R-free | 0.26280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nc2 5imk 6m0j |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.702 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.28) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.540 | 49.500 | 3.370 |
| High resolution limit [Å] | 3.200 | 10.120 | 3.200 |
| Rmerge | 0.153 | 0.037 | 1.261 |
| Rmeas | 0.157 | 0.038 | 1.296 |
| Rpim | 0.036 | 0.009 | 0.296 |
| Total number of observations | 18377 | 83417 | |
| Number of reflections | 30769 | 1131 | 4397 |
| <I/σ(I)> | 20.3 | 50.7 | 3.2 |
| Completeness [%] | 100.0 | 99.2 | 100 |
| Redundancy | 18.5 | 16.2 | 19 |
| CC(1/2) | 0.999 | 1.000 | 0.865 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.1 M (NH4)2SO4 0.1 M TRIS pH7.5 20% (w/v) PEG1500 |
