7FGL
The complex structure of PHF core domain peptide of tau, VQIVYK, and antibody's Fab domain.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2019-08-22 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5406 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.980, 70.720, 118.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.080 - 2.100 |
| R-factor | 0.20317 |
| Rwork | 0.200 |
| R-free | 0.26962 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6lra |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.539 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.080 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.060 | 0.364 |
| Number of reflections | 28351 | 28351 |
| <I/σ(I)> | 10.6 | |
| Completeness [%] | 99.4 | |
| Redundancy | 3.47 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 19% PEG3350, 20% Glycerol, 0.2M Ammonium iodide, 0.01M Tris-HCl |
