7FF9

Pseudomonas aeruginosa Virulence Factor Regulator with cAMP ligand and Cl(triethylphosphine)gold(I)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	200
Detector technology	PIXEL
Collection date	2019-09-22
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.9537
Spacegroup name	P 1
Unit cell lengths	49.738, 87.615, 104.973
Unit cell angles	79.04, 77.87, 75.40

Refinement procedure

Resolution	46.530 - 2.400
R-factor	0.2658
Rwork	0.264
R-free	0.29270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2oz6
RMSD bond length	0.010
RMSD bond angle	1.581
Data reduction software	HKL-3000
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.530	2.486
High resolution limit [Å]	2.400	2.400
Number of reflections	121837	6402
<I/σ(I)>	10.68
Completeness [%]	98.4
Redundancy	3
CC(1/2)	0.994	0.807

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	2.6M Ammonium Sulphate, 0.1M Bicine pH 9.0