7FF5

The crystal structure of Ruminiclostridium cellulolyticum Phosphocarrier

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 41 21 2
Unit cell lengths	96.004, 96.004, 89.836
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.370
R-factor	0.2156
Rwork	0.214
R-free	0.25620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mo1
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (v1.16)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.450
High resolution limit [Å]	2.370	2.370
Rmerge	0.015	0.061
Number of reflections	17552	1694
<I/σ(I)>	36.4
Completeness [%]	99.6
Redundancy	1.9
CC(1/2)	0.999	0.988

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		288	0.2 M Sodium sulfate, 20% w/v PEG3350, 10% w/v Ethylene glycol