7FF5
The crystal structure of Ruminiclostridium cellulolyticum Phosphocarrier
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-01-07 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97915 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 96.004, 96.004, 89.836 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.370 |
R-factor | 0.2156 |
Rwork | 0.214 |
R-free | 0.25620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mo1 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX (v1.16) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.450 |
High resolution limit [Å] | 2.370 | 2.370 |
Rmerge | 0.015 | 0.061 |
Number of reflections | 17552 | 1694 |
<I/σ(I)> | 36.4 | |
Completeness [%] | 99.6 | |
Redundancy | 1.9 | |
CC(1/2) | 0.999 | 0.988 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 288 | 0.2 M Sodium sulfate, 20% w/v PEG3350, 10% w/v Ethylene glycol |