7FDA
CryoEM Structure of Reconstituted V-ATPase, state1
EM specimen
Vitrification | Yes |
Instrument | HOMEMADE PLUNGER |
EM Data Collection
Microscope | TFS KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Energy Filter | GIF Bioquantum |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 4.2 |
Software | cryoSPARC,PHENIX,EPU,Coot |
Symmetry | C1 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |