Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.205, 83.151, 156.083 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.410 - 3.300 |
| R-factor | 0.2728 |
| Rwork | 0.271 |
| R-free | 0.30620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y12 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.539 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.410 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.120 | 1.310 |
| Number of reflections | 15808 | 1191 |
| <I/σ(I)> | 22.75 | |
| Completeness [%] | 97.2 | |
| Redundancy | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2M Ammonium acetate, 0.1M HEPES pH 7.5, 25% PEG 3350 |
