1Y12

Structure of a hemolysin-coregulated protein from Pseudomonas aeruginosa

Summary for 1Y12

• Entry DOI: 10.2210/pdb1y12/pdb
• Descriptor: hypothetical protein PA0085 (2 entities in total)
• Functional Keywords: pseudomonas aeruginosa, hemolysin-corregulation, structural genomics, psi, protein structure initiative, midwest center for structural genomics, mcsg, unknown function
• Biological source: Pseudomonas aeruginosa PAO1
• Total number of polymer chains: 3
• Total formula weight: 53964.84

Authors

Cuff, M.E.,Zhou, M.,Joachimiak, A.,Midwest Center for Structural Genomics (MCSG) (deposition date: 2004-11-16, release date: 2005-01-25, Last modification date: 2024-11-20)

Primary citation

Mougous, J.D.,Cuff, M.E.,Raunser, S.,Shen, A.,Zhou, M.,Gifford, C.A.,Goodman, A.L.,Joachimiak, G.,Ordonez, C.L.,Lory, S.,Walz, T.,Joachimiak, A.,Mekalanos, J.J.
A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus.
Science, 312:1526-1530, 2006

PubMed Abstract: Bacterial pathogens frequently use protein secretion to mediate interactions with their hosts. Here we found that a virulence locus (HSI-I) of Pseudomonas aeruginosa encodes a protein secretion apparatus. The apparatus assembled in discrete subcellular locations and exported Hcp1, a hexameric protein that forms rings with a 40 angstrom internal diameter. Regulatory patterns of HSI-I suggested that the apparatus functions during chronic infections. We detected Hcp1 in pulmonary secretions of cystic fibrosis (CF) patients and Hcp1-specific antibodies in their sera. Thus, HSI-I likely contributes to the pathogenesis of P. aeruginosa in CF patients. HSI-I-related loci are widely distributed among bacterial pathogens and may play a general role in mediating host interactions.

PubMed: 16763151
DOI: 10.1126/science.1128393

Experimental method

X-RAY DIFFRACTION (1.95 Å)