7FAK
Co-crystal Structure of Toxoplasma gondii Prolyl tRNA Synthetase (TgPRS) in complex with L96 and L-pro
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-28 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.9795 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 113.619, 74.230, 74.648 |
Unit cell angles | 90.00, 111.05, 90.00 |
Refinement procedure
Resolution | 60.811 - 1.899 |
R-factor | 0.1829 |
Rwork | 0.181 |
R-free | 0.21640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6aa0 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.309 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.15) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.811 | 1.940 |
High resolution limit [Å] | 1.890 | 1.890 |
Rmeas | 0.125 | 0.890 |
Number of reflections | 45141 | 2157 |
<I/σ(I)> | 8.7 | 1.3 |
Completeness [%] | 98.6 | 94.9 |
Redundancy | 6.6 | 5.7 |
CC(1/2) | 1.000 | 0.500 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Morpheus G2 |