7FAJ
CARM1 bound with compound 43
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-11-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97915 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 75.296, 99.339, 207.141 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 60.925 - 2.245 |
R-factor | 0.193692820483 |
Rwork | 0.191 |
R-free | 0.23982 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6izq |
RMSD bond length | 0.008 |
RMSD bond angle | 0.863 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.300 | 2.300 |
High resolution limit [Å] | 2.245 | 2.250 |
Rmerge | 0.074 | 0.656 |
Number of reflections | 74529 | 74529 |
<I/σ(I)> | 14.5 | |
Completeness [%] | 99.3 | 99.9 |
Redundancy | 13.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 22-27% PEG 3350, 0.15M sodium malate, pH 7.0 |