7F9N

Crystal structure of the variable region of Plasmodium RIFIN #4 (PF3D7_1000500) in complex with LAIR1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2018-10-02
Detector	SDMS
Wavelength(s)	0.97894
Spacegroup name	P 41 21 2
Unit cell lengths	73.435, 73.435, 341.046
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.670 - 3.000
R-factor	0.2674
Rwork	0.264
R-free	0.33020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kgr
RMSD bond length	0.016
RMSD bond angle	1.394
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.110
High resolution limit [Å]	3.000	3.000
Number of reflections	19913	19913
<I/σ(I)>	21.7
Completeness [%]	100.0
Redundancy	21
CC(1/2)	0.997	0.637

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M di-ammonium hydrogen citrate, 20% w/v PEG 3,350