7F9M
Crystal structure of the variable region of Plasmodium RIFIN #4 (PF3D7_1000500) in complex with LAIR1 (with T67L, N69S and A77T mutations)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-07-12 |
| Detector | SDMS |
| Wavelength(s) | 1.03662 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 73.782, 73.782, 345.328 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.520 - 2.900 |
| R-factor | 0.2568 |
| Rwork | 0.254 |
| R-free | 0.30990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kgr |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.330 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 22348 | 22348 |
| <I/σ(I)> | 22.72 | |
| Completeness [%] | 100.0 | |
| Redundancy | 17.6 | |
| CC(1/2) | 0.995 | 0.606 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M citric acid pH 3.7, 8% w/v polyethylene glycol 1,000 |
