7F9B
Homo sapiens Prolyl-tRNA Synthetase (HsPRS) in Complex with L-proline and compound L95
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-18 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9799 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.730, 90.960, 83.490 |
| Unit cell angles | 90.00, 110.03, 90.00 |
Refinement procedure
| Resolution | 45.480 - 1.995 |
| R-factor | 0.1914 |
| Rwork | 0.189 |
| R-free | 0.23560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vad |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASES |
| Refinement software | PHENIX (1.15rc1_3423) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.480 | 2.110 |
| High resolution limit [Å] | 1.995 | 1.995 |
| Rmerge | 0.118 | 0.928 |
| Rmeas | 0.127 | 1.008 |
| Number of reflections | 66905 | 10924 |
| <I/σ(I)> | 9.9 | |
| Completeness [%] | 98.4 | 94.7 |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 0.1 M HEPES, 1.5 M CaCl2, 20% PEG 3350 |
