7F8N
Human pannexin-1 showing a conformational change in the N-terminal domain and blocked pore
EM specimen
| Vitrification | Yes |
| Instrument | LEICA KF80 |
EM Data Collection
| Microscope | JEOL CRYO ARM 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | In-column Omega Filter |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.4 |
| Number of Particle | 422244 |
| Software | EMAN2,RELION |
| Symmetry | C7 |
| CTF correction | PHASE FLIPPING ONLY |
