7F8J
Cryo-EM structure of human pannexin-1 in a nanodisc
EM specimen
| Vitrification | Yes |
| Instrument | LEICA KF80 |
EM Data Collection
| Microscope | JEOL CRYO ARM 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | In-column Omega Filter |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.6 |
| Number of Particle | 1533798 |
| Software | EMAN2,RELION,PHENIX,CTFFIND |
| CTF correction | PHASE FLIPPING ONLY |
