7F79

Crystal structure of glutamate dehydrogenase 3 from Candida albicans in complex with alpha-ketoglutarate and NADPH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-31
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	96.279, 155.224, 98.807
Unit cell angles	90.00, 95.50, 90.00

Refinement procedure

Resolution	44.800 - 2.700
R-factor	0.2013
Rwork	0.199
R-free	0.24720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xvx
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MoRDa
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.800
High resolution limit [Å]	2.700	5.810	2.700
Rmerge	0.156	0.054	0.984
Rmeas	0.185	0.064	1.167
Rpim	0.098	0.034	0.620
Total number of observations	274007
Number of reflections	79980	8088	7950
<I/σ(I)>	3.8
Completeness [%]	98.5	98	98.7
Redundancy	3.4	3.5	3.4
CC(1/2)		0.985	0.528

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	0.2 M DL-Malic acid, pH 7.0, and 20% PEG 3350